Electrophoresis: chii icho, chii chaicho uye kuti inoitwa sei

Zvemukati

• Chii ichochi
• Zvinoitwa sei
• Mhando dze electrophoresis
• 1. Hemoglobin electrophoresis
• 2. Mapuroteni electrophoresis

Electrophoresis inzira yerabhoritari inoitwa nechinangwa chekuparadzanisa mamorekuru zvinoenderana nehukuru hwayo uye mari yemagetsi kuitira kuti kuongororwa kwezvirwere zviitwe, kutaura kweproteinheni kunogona kusimbiswa kana microorganisms inogona kuzivikanwa.

Electrophoresis iri nyore uye yakaderera-mutengo maitiro, ari kushandiswa mumarabhoritari maitiro uye mune ekutsvaga mapurojekiti. Zvinoenderana nechinangwa che electrophoresis, zvinogona kuve zvakakosha kuita mimwe bvunzo uye bvunzo kuitira kuti uwane chirwere, semuenzaniso.

Chii ichochi

Electrophoresis inogona kuitirwa zvinangwa zvakati wandei, zvese mumapurojekiti ekutsvagisa uye pakuongorora, nekuti iri nyore uye yakachipa-mutengo nzira.Nekudaro, electrophoresis inogona kuitwa kune:

• Ziva hutachiona, fungi, mabhakitiriya uye zvipembenene, nechishandiso ichi chiri kunyanya kuwanikwa muzvirongwa zvekutsvaga.
• Paternity bvunzo;
• Tarisa kutaura kwemapuroteni;
• Ziva shanduko, uchibatsira mukuongororwa kwemaleukemias, semuenzaniso;
• Ongorora mhando dzekutenderera hemoglobin, zvichikosha mukuongororwa kwejeko masero anemia;
• Ongorora huwandu hweeprotein aripo muropa.

Zvinoenderana nechinangwa che electrophoresis, zvinogona kuve zvakakosha kuita mamwe mabvunzo ekuwedzera kuti chiremba apedze kuongororwa.

Zvinoitwa sei

Kuti uite electrophoresis zvakafanira iyo gel, iyo inogona kunge iri ye polyacrylamide kana agarose zvinoenderana nechinangwa, electrophoresis buffer uye vat, molecular weight marker uye dhayi ye fluorescent, kuwedzera kune UV kana LED michina yemwenje, inozivikanwa se transilluminator .

Mushure mekugadzira gel, chinhu chakatarwa chinofanirwa kuiswa kuti dziite matsime ari mujeri, anonyanya kudaidzwa kuti muzinga, uye rega gel riise. Kana gel ragadzirira, ingoisa izvo zvinhu kumatsime. Kune izvi, mamorekuru uremu mucherechedzo unofanirwa kuiswa mune rimwe rematsime, yakanaka control, chinova chinhu chinozivikanwa kuti chii, kudzora kwakashata, kunovimbisa huchokwadi hwekupindura, uye iwo masampuro achaongororwa. Zvese sampuli zvinofanirwa kuve zvakasanganiswa ne fluorescent dhayi, sezvo nenzira iyi zvinokwanisika kuona mabhanhire pane iyo transilluminator.

Iyo gel ine masampuli inofanirwa kuiswa mune electrophoresis vat, iyo ine yakasarudzika buffer solution, uyezve chishandiso ichivhurwa kuitira kuti kuve nemagetsi emagetsi uye, nekudaro, musiyano ungangoitika, izvo zvakakosha pakupatsanurwa kwezvinhu kumutoro wavo uye kukura. Iyo electrophoretic yekumhanya nguva inosiyana zvinoenderana nechinangwa chemaitiro, uye inogona kutora kusvika paawa rimwe.

Mushure menguva yakatarwa, zvinokwanisika kuona mhedzisiro yeelectrophoretic inomhanya kuburikidza neiyo transilluminator. Kana iyo gel ikaiswa pasi peUV kana mwenje weLEM, zvinokwanisika kuona mabhendi mabhendi: iyo yakakura mamorekuru, iyo shoma painofamba, ichiswedera padyo netsime, nepo iyo yakareruka mamorekuru, ihwo hunowedzera mukana wekufamba.

Kuti chiitiko chisimbiswe, zvinofanirwa kuti zvisungo zvekutonga kwakanaka zvionekwe uye kuti mukutonga kwakashata hapana chinoonekwa, nekuti zvikasadaro chiratidzo chekuti paive nekusviba, uye maitiro ese anofanira kudzokororwa.

Mhando dze electrophoresis

Electrophoresis inogona kuitirwa zvakasiyana zvinangwa uye, maererano nechinangwa chayo, mhando dzinoverengeka dzejel dzinogona kushandiswa, yakanyanya kuve polyacrylamide uye agarose.

Electrophoresis yekuona hutachiona hwakajairika kuitwa mumarabhoritari ekutsvagisa, zvisinei, nekuda kwechinangwa chekuongorora, electrophoresis inogona kushandiswa kuona zvirwere zvehematological nezvirwere zvinoshanduka nekuwedzera kwehuwandu hwemapuroteni, ari iwo makuru marudzi e electrophoresis:

1. Hemoglobin electrophoresis

Hemoglobin electrophoresis inzira yerabhoritari inoitwa kuratidza mhando dzakasiyana dzehemoglobin inotenderera muropa, zvichiita kuti zvikwanise kuona huvepo hwezvirwere zvine chekuita nehemoglobin synthesis. Mhando yehemoglobin inozivikanwa nenzira yeelectrophoresis pane imwe pH, iri pakati pe8.0 ne9.0, ine pateni yemabhandi iri kuongororwa inogona kufananidzwa neyakajairika pateni, ichibvumira kuzivikanwa kwekuvapo kweasina kujairika hemoglobins.

Izvo zvakagadzirirwa: Hemoglobin electrophoresis inoitwa kuongorora nekuongorora zvirwere zvine chekuita nehemoglobin synthesis, senge sickle cell anemia uye hemoglobin C chirwere, mukuwedzera mukubatsira kusiyanisa thalassemia. Dzidza kududzira hemoglobin electrophoresis.

2. Mapuroteni electrophoresis

Protein electrophoresis bvunzo yakakumbirwa nachiremba kuti aongorore huwandu hwemapuroteni anotenderera muropa uye, nekudaro, kuziva zvirwere. Muedzo uyu unoitwa kubva muyero yeropa, iri centrifuged kuti iwane plasma, inova chikamu cheropa, chinosanganisira, pakati pezvimwe zvinhu, zvemapuroteni.

Mushure meelectrophoresis, pateni yemabhande inogona kutaridzika uye, zvichidaro, girafu umo huwandu hwesekisheni yega yega yemapuroteni inoratidzwa, ichikosha pakuongororwa.

Izvo zvakagadzirirwa: Protein electrophoresis inobvumidza chiremba kuti aongorore kuitika kweakawanda myeloma, kupera mvura mumuviri, cirrhosis, kuzvimba, chirwere chechiropa, pancreatitis, lupus uye hypertension zvinoenderana nebhendi pateni uye girafu inoratidzwa mumushumo wekuongorora.

Nzwisisa kuti zvinoitwa sei uye kuti unganzwisisa sei mhedzisiro yeprotein electrophoresis.