Protein Electrophoresis: ndeyei uye kuti unganzwisisa sei mhedzisiro

Zvemukati

• Chii ichochi
• Zvinoitwa sei
• Maitiro ekunzwisisa mhedzisiro
• Albumin
• Arufa-1-globulin
• Arufa-2-globulin
• Beta-1-globulin
• Beta-2-globulin
• Gamma-globulin

Protein electrophoresis bvunzo yakakumbirwa nachiremba nechinangwa chekuongorora zvirwere zvinogona kukonzera shanduko muhuwandu hwemapurotini anotenderera muropa, ichionekwa seimwe yemiedzo mikuru yakakumbirwa kuferefetwa nekuongororwa kweakawanda myeloma.

Kuongorora uku kunoitwa kubva muyero yeropa, iyo inoitisa centrifugation maitiro kuti uwane plasma yeropa, umo mapuroteni anowanikwa. Aya mapuroteni anobva atora nzira yekuparadzanisa zvinoenderana nechaji yavo yemagetsi uye uremu hwemaseru, zvichitungamira mukuumbwa kwechikwata chechikwata uye, zvichidaro, girafu yakakosha kududzirwa kwekuongororwa nachiremba.

Mapuroteni anoongororwa mune bvunzo iyi akakosha kuti mashandiro akanaka emuviri, nekuti anoshanda pane immune system, mune coagulation maitiro uye metabolic reaction, mukuwedzera pakukwanisa kutakura mamwe mamorekuru kuenda kune yavo saiti yekuita. Nekudaro, shanduko mukukadzika kwavo inogona kuratidza zvirwere. Pakati pemapuroteni akaongororwa pane albumin, alpha-glycoproteins, beta-glycoproteins uye gamma-glycoproteins.

Chii ichochi

Mapuroteni electrophoresis anokumbirwa nachiremba kuti atarise huwandu hweprotein mumuviri uye, nekudaro, ongorora shanduko dzinogona kuitika nezvirwere, kugona kutanga kurapwa nekukurumidza, kana zvirizvo. Mimwe yemamiriro ezvinhu anga arayirwe nachiremba uye mapuroteni electrophoresis ndipo paine zviratidzo nezviratidzo zvinoratidza zve:

• Kupera mvura mumuviri;
• Akawanda myeloma;
• Kuzvimba;
• Cirrhosis;
• Systemic lupus erythematosus;
• Hypertension;
• Ascites;
• Glomerulonephritis;
• Cushing's syndrome;
• Emphysema;
• Zvirwere zvechiropa;
• Kupererwa neropa;
• Pancreatitis.

Pamusoro pezviitiko izvi, bvunzo iyi inogona kukumbirwa kana munhu achirapwa estrogen kana aine nhumbu, sezvo mumamiriro ezvinhu aya panogona kunge paine shanduko mumaprotein mazinga, zvakakosha kutarisa mapuroteni akachinjwa uye kutora matanho uye kudzosera kumashure mamiriro acho ezvinhu.

Zvinoitwa sei

Mapuroteni electrophoresis anoitwa nekutora muyero weropa kubva kumunhu nehunyanzvi hwakadzidziswa uye hapana gadziriro inodiwa. Muenzaniso unowanikwa unotumirwa murabhoritari kuitira kuti pave nekuparadzaniswa pakati pemasero matsvuku eropa neplasma. Mune mamwe mamiriro ezvinhu, maawa makumi maviri nemana ekuunganidza weti inogona kuitwa kuti utarise huwandu hweprotein yakaburitswa mumuti mukati mezuva, izvo zvinonyanya kukumbirwa nachiremba kana matambudziko eitsvo achifungidzirwa.

Iyo plasma inozoiswa muagarose gel kana cellulose acetate pamwe chete nedhayi uye mucherechedzo wepuroteni rega rega uyezve magetsi anoshandiswa kuitira kukurudzira kupatsanurwa kwemapuroteni zvinoenderana nekwaniso yavo yemagetsi., Saizi uye mamorekuru uremu. Mushure mekuparadzaniswa, mapuroteni anogona kutariswa nenzira yechikwata chechikwata, zvichiratidza kuvepo kana kusavapo kwemapuroteni.

Zvino, mapuroteni aya akaverengwa mune chimwe chigadzirwa, chinonzi densitometer, umo kusunganidzwa kwemapuroteni muropa kunoongororwa, neepesenti kukosha uye kukosha kwakakwana kwechikamu chimwe nechimwe cheprotein chiri kuratidzwa mushumo, kuwedzera kune girafu, izvo zvakakosha kuti unzwisise zvirinani nachiremba uye nemurwere wemhedzisiro yemuedzo.

Maitiro ekunzwisisa mhedzisiro

Mhedzisiro yeprotein electrophoresis bvunzo inofanirwa kududzirwa nachiremba, uyo anoongorora kukosha kwakakwana uye kwakaringana kwemapuroteni, kuwedzera kune iyo graph inoburitswa mushumo.

Mhedzisiro yacho, zvikamu zveprotein zvinoratidzwa, ndiko kuti, hunhu hunowanikwa mualbumin, alpha-1-globulin, alpha-2-globulin, beta-1-globulin, beta-2-globulin uye gamma-globulin. Nezve iyo bhendi pateni, kazhinji haina kuburitswa mushumo, ichingosara murabhoritari uye inowanika kuna chiremba.

Albumin

Albumin ipuroteni yeplasma iripo yakawanda uye inogadzirwa muchiropa, ichiita mabasa akasiyana siyana, sekutakura mahormone, mavitamini uye zvinovaka muviri, kudzora pH uye osmotic kutonga kwemuviri. Iyo synthesis yealbumin muchiropa inoenderana nehutano hwemunhu, huwandu hwekutenderera mahormone uye ropa pH. Nekudaro, huwandu hwealbumin muprotein electrophoresis inoratidza huwandu hwenzvimbo yemunhu uye hunobvumidza kuona shanduko dzinogona kuitika muchiropa kana neitsvo.

Referendi kukosha muelectrophoresis (inogona kusiyana zvichienderana nerabhoritari): 4.01 kusvika 4.78 g / dL; 55.8 kusvika 66.1%

Yakawedzera albumin: Kuwedzera kweAlbumin mazinga kunoitika zvakanyanya semhedzisiro yekushaya mvura, asi kwete nekuti paive nekuwedzera mukugadzirwa kweprotein iyi, asi nekuti huwandu hwemvura hudiki uye nekudaro, huwandu hweropa, nekudaro, akakwira mwero wealbumin ari yakasimbiswa.

Yakaderera albumin: Albumin inoonekwa seyakaipisisa chikamu cheprotein, ndiko kuti, mumamiriro ekuzvimba, pane kuderera kwemazinga ealbumin. Nekudaro, kuderera kwealbumin kunogona kuitika muzviitiko zvechirwere cheshuga mellitus, hypertension, edema, ascites, kushomeka kwekudya zvinovaka muviri uye cirrhosis, umo chiropa chakakanganiswa uye albumin synthesis inova isina simba.

Dzidza zvakawanda nezve albumin.

Arufa-1-globulin

Iyo alpha-1-globulin chidimbu ine mapuroteni akati wandei, makuru acho ari iwo alpha-1-asidhi glycoprotein (AGA) uye iyo alpha-1-antitrypsin (AAT). AGA inotora chikamu mukuumbwa kweiyo collagen fibers uye inokonzeresa kudzora zviitiko zvehutachiona uye zvipembenene, nekudaro iine chinzvimbo chakakosha mukushanda chaiko kwema immune system. Kunge AGA, AAT zvakare ine kukosha kukuru mune immune system.

Referendi kukosha muelectrophoresis (inogona kusiyana zvichienderana nerabhoritari): 0,22 kusvika 0,41 g / dL; 2.9 kusvika 4.9%

Yakawedzera alpha-1-globulin: Kuwedzera kwemapuroteni muchikamu chino kunoitika zvakanyanya nekuda kwekuzvimba uye hutachiona. Nekudaro, akakwirisa ealpha-1-globulin inogona kuratidza neoplasms, Cushing's syndrome, arthritis, nhumbu uye vasculitis, mukuwedzera pakukwanisa kuwedzera semhedzisiro yekurapa nemaestrogens kana corticosteroids.

Kuderera mu alpha-1-globulin: Kuderera kunogona kuitika semhedzisiro yephrotic syndrome, chirwere chakakomba chechiropa, emphysema, cirrhosis uye hepatocellular carcinoma.

Arufa-2-globulin

Iyo alpha-2-globulin chidimbu inoumbwa nemapuroteni makuru matatu: iyo ceruloplasmin (CER), a haptoglobin (hpt) uye iyo macroglobulin (AMG), ine huwandu hunogona kuwedzera semhedzisiro yekuzvimba uye hutachiona maitiro.

Ceruloplasmin ipuroteni inogadzirwa nechiropa uye ine huwandu hwakawanda hwemhangura mukuumbwa kwayo, iyo inobvumira kuti iite zvimwe zvinoitika mumuviri. Uye zvakare, CER yakakosha mukuita kwekusanganisa iron mu transferrin, inova puroteni inoitisa kutakura simbi mumuviri. Kunyangwe ichionekwawo seyakanyanya chikamu mapuroteni, mazinga eCER anononoka kusimuka.

Haptoglobin inoitisa kusunga kutenderera hemoglobin uye, nekudaro, kusimudzira kudzikisira kwayo uye kubvisa kubva mukutenderera. Macroglobulin, kune rumwe rutivi, ndeimwe yemapuroteni eplasma uye ane basa rekugadzirisa kuzvimba uye immune immune, mukuwedzera pakutakura mapuroteni akareruka, peptides, uye nekugadzirisa kusanganisa kwemapuroteni eplasma nechiropa.

Referendi kukosha muelectrophoresis (inogona kusiyana zvichienderana nerabhoritari): 0.58 kusvika 0.92 g / dL; 7.1 kusvika 11.8%

Yakawedzera alpha-2-globulin: Kuwedzera kwemapuroteni muchikamu ichi kunogona kuratidza nephrotic syndrome, chirwere chaWilson, kushomeka kwechiropa, chakaparadzirwa intravascular coagulation uye cerebral infarction, mukuwedzera pakukwanisa kuwedzera nekuda kweiyo estrogen kurapwa.

Kuderera mu alpha-2-globulin: Kuderera kwematanho eproteinini kunogona kuitika nekuda kwehemolytic anemias, pancreatitis uye zvirwere zvemapapu.

Beta-1-globulin

THE transerrin ndiyo protini huru yebeta-1-globulin chidimbu uye inoitisa kutakurwa kwesimbi kunzvimbo dzakasiyana mumuviri. Pamusoro peiyo huwandu hunogona kuongororwa muprotein electrophoresis, iko kusangana kweiyo transferrin muropa kunogona kuongororwa mune yakajairwa bvunzo yeropa. Ziva kuziva iyo transferrin bvunzo.

Referendi kukosha muelectrophoresis (inogona kusiyana zvichienderana nerabhoritari): 0.36 kusvika 0.52 g / dL; 4.9 kusvika 7.2%

Kuwedzera kwe beta-1-globulin: Iko kuwedzera kunoitika muzviitiko zvekushomeka kwesimbi anemia, nhumbu, jaundice, hypothyroidism uye chirwere cheshuga.

Kuderera mu beta-1-globulin: Kuderera kweichi chidimbu chemapuroteni hakuwanzo kuitika, zvisinei, zvinogona kucherechedzwa mune zvisingaperi maitiro.

Beta-2-globulin

Muchikamu chino pane mapuroteni maviri makuru, iyo beta-2-microglobulin (BMG) uye iyo C-inoshanda mapuroteni (CRP). BMG mucherechedzo wemaitiro emagetsi, zvichikosha pakutsvaga mamota e-lymphocytic, semuenzaniso, mukuwedzera pakukwanisa kushandiswa mune zvekurapa nechinangwa chekuperekedza murwere wekenza, kuitira kuona kana kurapwa kuri kushanda. CRP ipuroteni yakakosha mukucherechedzwa kwehutachiona uye kuzvimba, nekuti ndiyo inowanzo shanduka muzvikamu zvayo.

Referendi kukosha muelectrophoresis (inogona kusiyana zvichienderana nerabhoritari): 0,22 kusvika 0,45 g / dL; 3.1 kusvika 6.1%

Kuwedzera kwe beta-2-globulin: Iko kuwedzera kunogona kuitika mune izvo zviitiko zvezvirwere zvine hukama nema lymphocyte, kuzvimba uye hutachiona.

Kuderera mu beta-2-globulin: Iko kuderera kunogona kunge kuri nekuda kwechinetso chechiropa, icho chinodzivirira kusanganisa kweaya mapuroteni.

Gamma-globulin

Muchikamu chino cheprotein electrophoresis inowanikwa immunoglobulins, ayo ari mapuroteni anoitisa kudzivirira kweiyo muviri. Nzwisisa mashandiro anoita immune system.

Referendi kukosha muelectrophoresis (inogona kusiyana zvichienderana nerabhoritari): 0.72 kusvika 1.27 g / dL; 11.1 kusvika 18.8%

Gamma-globulin kuwedzera: Iko kuwedzera kwegamma-globulin chidimbu chemapuroteni kunoitika mukutarisana nehutachiona, kuzvimba uye autoimmune hosha, senge rheumatoid arthritis. Uye zvakare, panogona kuve nekuwedzera mune kesi ye lymphoma, cirrhosis uye yakawanda myeloma.

Gamma-globulin inoderera: Kazhinji, immunoglobulin mazinga anoderedzwa kana paine kushomeka mune immune system nekuda kwezvirwere zvisingaperi, semuenzaniso.